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tween 20 tbst washes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tween 20 tbst washes
    Tween 20 Tbst Washes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/washes+with+tbst/Tween+20/pm41955091-127-4-17
    Average 99 stars, based on 1 article reviews
    tween 20 tbst washes - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: LDHA associated with telomerase-related genes promotes tumorigenesis and progression of lung adenocarcinoma
    Article Snippet: Membranes were blocked with 5% skim milk in TBST (Tris-buffered saline with 0.1% Tween-20) at room temperature for 1 h, then incubated overnight at 4 °C with primary antibodies against: LDHA (Rabbit polyclonal; EpyZime, Wuhan, China;1:2000 dilution), β-tubulin (Mouse monoclonal; Proteintech,1:10,000 dilution). .. After three washes with TBST (10 min each), membranes were incubated for 1 h at room temperature with species-matched secondary antibodies: HRP-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG (both from Thermo Fisher Scientific; 1:5000 dilution) were used as secondary antibodies. .. The immunoreactive bands were visualized using Superstar ECL Plus substrate (Boster) and imaged on a ChemiScope 6000 chemiluminescence imaging system.

    Article Title: Inhibitory Mechanism of Buyang Huanwu Decoction on AGE/RAGE Pathway in Membranous Nephropathy: Integration of Network Pharmacology and Cell Model Validation
    Article Snippet: .. After three washes with TBST (10 minutes each), the membranes were incubated at room temperature for 1 hour with the HRP-conjugated secondary antibodies (1:10,000, 31460, Thermo Fisher Scientific). .. Protein bands were visualized using an enhanced chemiluminescence (ECL) reagent (Thermo Fisher Scientific), and band intensities were quantified using the ImageJ software.

    Article Title: Profiling the Pancreatic Cancer Secretome with Metabolic Glycoengineering.
    Article Snippet: Aliquots Jo urn al Pr e-p ro f 37 containing 20 μg of protein were then loaded on 4-15% gel for SDS-PAGE separation then transferred onto a nitrocellulose membrane using an iBlot 2 Dry Blotting System (Invitrogen, IB21001), Membranes were blocked in EveryBlot Blocking Buffer (Bio-Rad, 12010020) for 5 min and then incubated with ApoA1 polyclonal antibody (1:1,000; Thermo Fisher Scientific, PA529557) and DCTPP1 polyclonal antibody (1:10,000; Thermo Fisher Scientific, A305-794A-T) for 1 h at room temperature with gentle agitation. .. Following washes with TBST (3 x 10 min), membranes were incubated with goat anti-rabbit IgG-HRP secondary antibody (1:10,000 in TBST; Thermo Fisher Scientific, 31460) for 1 h at room temperature. .. Protein bands were visualized using Chemiluminescent substrate (Thermo Fisher Scientific, 34095), and imaged using a GE Healthcare ImageQuant LAS 4000 series instrument.

    Article Title: Elucidation of ACAT1's role in hepatic ischemia-reperfusion injury: TFEB-mediated mitophagy and ferroptosis modulation as therapeutic targets
    Article Snippet: After washing the cells with PBS, they were treated with 0.1% Triton (L885651, Macklin Biochemical Co., Ltd., Shanghai, China) for 15 min. After two additional PBS washes, the cells were incubated in PBS containing 15% FBS at 5 °C for 4 min. Next, the cells were incubated overnight at 4°C with a rabbit anti-LC3 antibody (PA1-46286, 1:50; Thermo Fisher Scientific Inc.). .. After three washes with TBST (TBS containing 1% Tween-20), the cells were incubated with sheep anti-rabbit Alexa Fluor® 488 secondary antibody (A-11008, Thermo Fisher Scientific Inc.) at room temperature for 2 h. A second staining with MitoTracker Red was performed before analysis using confocal laser scanning microscopy. .. Specific regions of interest were selected for fluorescence intensity measurement, and image processing, quantification, and positive cell enumeration were carried out using ImageJ software.

    Article Title: LDHA associated with telomerase-related genes promotes tumorigenesis and progression of lung adenocarcinoma.
    Article Snippet: Membranes were blocked with 5% skim milk in TBST (Tris-buffered saline with 0.1% Tween-20) at room temperature for 1 h, then incubated overnight at 4 °C with primary antibodies against: LDHA (Rabbit polyclonal; EpyZime, Wuhan, China;1:2000 dilution), β-tubulin (Mouse monoclonal; Proteintech,1:10,000 dilution). .. After three washes with TBST (10 min each), membranes were incubated for 1 h at room temperature with speciesmatched secondary antibodies: HRP-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG (both from Thermo Fisher Scientific; 1:5000 dilution) were used as secondary antibodies. .. The immunoreactive bands were visualized using Superstar ECL Plus substrate (Boster) and imaged on a ChemiScope 6000 chemiluminescence imaging system.

    Article Title: Profiling the Pancreatic Cancer Secretome with Metabolic Glycoengineering.
    Article Snippet: Western blots were probed with anti-GBA monoclonal antibodies (1:1,000, Abcam, ab55080) 1 h at room temperature with gentle agitation. .. Following washes with TBST (3 x 10 min), membranes were incubated with goat anti-mouse IgG-HRP secondary antibody (1:10,000 in TBST; Thermo Fisher Scientific, 31430) for 1 h at room temperature. ..

    Imaging:

    Article Title: 3D “Emboli” Culture Models Epithelial Breast Cancer Cell Oxidative Mitochondrial Metabolism with Relevance for Lung Metastasis
    Article Snippet: .. Following three washes with TBST (TBS containing 0.05% Tween-20), appropriate secondary antibody was added for 1 hour, followed by three washes before scanning with the iBRIGHT 1500 Imaging System (Thermo Fischer Scientific). .. Antibodies were obtained from the following sources: Cell Signaling Technology (ID1, #23369S; ID3, #9837: RRID AB_2732885; E-cadherin, #3195; and vimentin, #46173) and DHSB (A-actin, # 12G10).

    Article Title: 3D “emboli” culture models epithelial breast cancer cell oxidative mitochondrial metabolism with relevance for lung metastasis
    Article Snippet: .. Following three washes with TBST (TBS containing 0.05% Tween-20), appropriate secondary antibody was added for 1 h followed by three washes before scanning with the iBRIGHT 1500 Imaging System (Thermo Fischer Scientific). .. Antibodies were obtained from the following sources: Cell Signaling Technology (ID1, #23369S; ID3, #9837: RRID AB_2732885; E-Cadherin, #3195; vimentin, #46173); DHSB (A-Actin, # 12G10).

    Staining:

    Article Title: Elucidation of ACAT1's role in hepatic ischemia-reperfusion injury: TFEB-mediated mitophagy and ferroptosis modulation as therapeutic targets
    Article Snippet: After washing the cells with PBS, they were treated with 0.1% Triton (L885651, Macklin Biochemical Co., Ltd., Shanghai, China) for 15 min. After two additional PBS washes, the cells were incubated in PBS containing 15% FBS at 5 °C for 4 min. Next, the cells were incubated overnight at 4°C with a rabbit anti-LC3 antibody (PA1-46286, 1:50; Thermo Fisher Scientific Inc.). .. After three washes with TBST (TBS containing 1% Tween-20), the cells were incubated with sheep anti-rabbit Alexa Fluor® 488 secondary antibody (A-11008, Thermo Fisher Scientific Inc.) at room temperature for 2 h. A second staining with MitoTracker Red was performed before analysis using confocal laser scanning microscopy. .. Specific regions of interest were selected for fluorescence intensity measurement, and image processing, quantification, and positive cell enumeration were carried out using ImageJ software.

    Confocal Laser Scanning Microscopy:

    Article Title: Elucidation of ACAT1's role in hepatic ischemia-reperfusion injury: TFEB-mediated mitophagy and ferroptosis modulation as therapeutic targets
    Article Snippet: After washing the cells with PBS, they were treated with 0.1% Triton (L885651, Macklin Biochemical Co., Ltd., Shanghai, China) for 15 min. After two additional PBS washes, the cells were incubated in PBS containing 15% FBS at 5 °C for 4 min. Next, the cells were incubated overnight at 4°C with a rabbit anti-LC3 antibody (PA1-46286, 1:50; Thermo Fisher Scientific Inc.). .. After three washes with TBST (TBS containing 1% Tween-20), the cells were incubated with sheep anti-rabbit Alexa Fluor® 488 secondary antibody (A-11008, Thermo Fisher Scientific Inc.) at room temperature for 2 h. A second staining with MitoTracker Red was performed before analysis using confocal laser scanning microscopy. .. Specific regions of interest were selected for fluorescence intensity measurement, and image processing, quantification, and positive cell enumeration were carried out using ImageJ software.



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